
Heejung Jung
Brenda Lopez
University of California
Riverside, CA
Jacob Swanson
Kevin Dover
Anne Kerber
Minnesota State University
Mankato, MN
Conduct of Research Report for TCRP Project F-30/
NCHRP Project 23-13(02)
Submitted May 2024

TCRP Web-Only Document 78/NCHRP Web-Only Document 410
Protecting Transportation Employees and the Traveling Public from Airborne Diseases
© 2024 by the National Academy of Sciences. National Academies of Sciences, Engineering, and Medicine and the graphical logo are trademarks of the National Academy of Sciences. All rights reserved.
Digital Object Identifier: 10.17226/28285
ACKNOWLEDGMENT
This work was sponsored by the Federal Transit Administration (FTA) in cooperation with the American Public Transportation Association (APTA) and the American Association of State Highway and Transportation Officials in cooperation with the Federal Highway Administration. It was conducted through the Transit Cooperative Research Program (TCRP), which is administered by the Transportation Research Board (TRB) of the National Academies of Sciences, Engineering, and Medicine.
NATIONAL COOPERATIVE HIGHWAY RESEARCH PROGRAM
Systematic, well-designed, and implementable research is the most effective way to solve many problems facing state departments of transportation (DOTs) administrators and engineers. Often, highway problems are of local or regional interest and can best be studied by state DOTs individually or in cooperation with their state universities and others. However, the accelerating growth of highway transportation results in increasingly complex problems of wide interest to highway authorities. These problems are best studied through a coordinated program of cooperative research.
Recognizing this need, the leadership of the American Association of State Highway and Transportation Officials (AASHTO) in 1962 initiated an objective national highway research program using modern scientific techniques—the National Cooperative Highway Research Program (NCHRP). NCHRP is supported on a continuing basis by funds from participating member states of AASHTO and receives the full cooperation and support of the Federal Highway Administration (FHWA), United States Department of Transportation, under Agreement No. 693JJ31950003.
COPYRIGHT INFORMATION
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Cooperative Research Programs (CRP) grants permission to reproduce material in this publication for classroom and not-for-profit purposes. Permission is given with the understanding that none of the material will be used to imply TRB, AASHTO, APTA, FAA, FHWA, FTA, GHSA, or NHTSA endorsement of a particular product, method, or practice. It is expected that those reproducing the material in this document for educational and not-for-profit uses will give appropriate acknowledgment of the source of any reprinted or reproduced material. For other uses of the material, request permission from CRP.
DISCLAIMER
The opinions and conclusions expressed or implied in this report are those of the researchers who performed the research. They are not necessarily those of the Transportation Research Board; the National Academies of Sciences, Engineering, and Medicine; or the program sponsors.
The Transportation Research Board does not develop, issue, or publish standards or specifications. The Transportation Research Board manages applied research projects which provide the scientific foundation that may be used by Transportation Research Board sponsors, industry associations, or other organizations as the basis for revised practices, procedures, or specifications.
The Transportation Research Board, the National Academies, and the sponsors of TCRP and NCHRP do not endorse products or manufacturers. Trade or manufacturers’ names appear herein solely because they are considered essential to the object of the report.
The information contained in this document was taken directly from the submission of the author(s). This material has not been edited by TRB.

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Monique R. Evans, Director, Cooperative Research Programs
Waseem Dekelbab, Deputy Director, Cooperative Research Programs, and Manager, National Cooperative Highway Research Program
Gwen Chisholm Smith, Manager, Transit Cooperative Research Program
Dianne S. Schwager, Senior Program Officer
Dajaih Bias-Johnson, Senior Program Assistant
Natalie Barnes, Director of Publications
Heather DiAngelis, Associate Director of Publications
Jennifer Correro, Assistant Editor
Ryan I. Daniel, St. Cloud Metro Bus, St. Cloud, MN (Chair)
Marla Blagg, Alameda-Contra Costa Transit District, Portland, OR
Kit Conway, Washington Metropolitan Area Transit Authority, Washington, DC
Avery Daugherty, Virginia Department of Rail and Public Transportation, Richmond, VA
Sean P. Fay, Johnstone & Lloyd, LLC, Salvisa, KY
Paul Goyette, USF Center for Urban Transportation Research, Spring Hill, FL
Amir Hessami, Texas A&M University-Kingsville, Kingsville, TX
Chukwuma A. Nnaji, Texas A&M University, College Station, TX
Lorena de Rodriguez, SSi, Inc., Phoenix, AZ
Brian L. Sherlock, Amalgamated Transit Union, Silver Spring, MD
Aydin Tabrizi, New York State Office of Information Technology Services, Albany, NY
Jean Landolt, FHWA Liaison
Brianna Butler, FTA Liaison
Marjorie Collins, FRA Liaison
Brian Thomas Alberts, APTA Liaison
Douglas M. Eaton, Jacobs Liaison
The team members are grateful to the students who participated in the passenger test to determine air exchange rate in the presence of passengers. We are grateful to the Los Angeles (LA) Metro for the loan of a bus. We are also grateful to Mike Todd and his team at Bourns College of Engineering Center for Environmental Research and Technology (CE-CERT) for the loan of a bus for the project. We appreciate Dr. Oliver Chang and his team for their loan of SMPS for the test. We appreciate Dan for driving the bus during on-road testing and Alain Gomes for providing mechanical services.
Students from the MSU Mankato Twin Cities Engineering team who contributed to the work in the report include John Nutt, Cosku Kaplan, Mohamed Abdi, Brendan Dykes, Sam Merchant, Yeng Moua, Joey Stam, and Tate Putman.
Students from the UCR who contributed to the work in the report include Evan Renck, Bryan Chen, and Megan Lee plus 10 CE-CERT students who volunteered for the stationary in-cabin bus testing.
By Dianne S. Schwager
Staff Officer
Transportation Research Board
This report provides information regarding strategies that mitigate exposure to airborne contagions by transportation employees and passengers on various modes, especially in buses. The COVID-19 pandemic increased awareness of airborne contagions and the importance of advanced air control systems and measures, which can lower the risk of airborne disease transmission. The primary audience for this research includes public transportation agencies (i.e., agencies that provide bus, rail, ferry, and paratransit services), departments of transportation, and practitioners.
Public transportation vehicles, in particular buses, can be high-risk environments since (1) passengers, who cannot socially distance themselves, are contained within a volume of air that may carry infectious diseases and (2) transportation employees are made vulnerable to airborne contagion, as their working environment inside a bus increases their risk of infection.
The research, which was jointly funded by the National Cooperative Highway Research Program (NCHRP) and the Transit Cooperative Research Program (TCRP), was conducted collaboratively by the University of California, Riverside, and Minnesota State University, Mankato. The objective of this research was to analyze and present strategies that mitigate exposure to airborne diseases to protect the health and well-being of transportation system employees while at work and to protect the traveling public. The research methodology included the following:
Chapter 1: Introduction, Research Objectives, and Overview of the Work Plan
Mitigation Methods on Public Transportation
Documented Exposure in Airplanes, Buses, and Trains
Aircraft Ventilation Strategies
Chapter 3: Experimental Studies with Transit Buses
Chapter 4: CFD Studies of Transit Buses
Chapter 5: CFD Studies of Subway and Tram Cars
Chapter 6: “Closed Box Model” can be used to Optimize Space to Reduce Virus
Chapter 7: Conclusions and Suggested Research
TCRP Web-Only Document 78/NCHRP Web-Only Document 410: Protecting Transportation Employees and the Traveling Public from Airborne Diseases presents the effectiveness of different strategies which will help agencies make more informed decisions regarding the health and well-being of their employees and the traveling public during periods of airborne contagion. Supplemental to the Web-Only Document is an Executive Summary, which can be accessed on the National Academies press website (nap.nationalacademies.org) by searching for Mitigating Exposure to Airborne Diseases for Public Transportation Passengers and Employees: Executive Summary.
Figure 1: Continuous aerosol generator (left), and cough generator (right)
Figure 2: Bus A test vehicle with cabin seat arrangement
Figure 3: Bus B Test Vehicle with cabin seat arrangement
Figure 4: Experimental project strategy flow chart
Figure 5: A completed barrier with CAD diagram of a barrier mounted to a seat in rear of the bus
Figure 6. CAD diagram layout of the barriers inside Bus A within the parallel ventilation
Figure 7: Simulated bus driving scenerio using a utility air circulating fan near the front door
Figure 8: Airflow throughout the standard transit bus ventilation system
Figure 9: Airflow in the transit bus with the parallel flow ventilation system
Figure 10: Installation of the lower ventilation system inside the bus
Figure 12: Parallel air flow system set up inside Bus A equipped with rear and front blowers
Figure 13: Suggested design of the parallel air flow system for production vehicles
Figure 14: Picture of one air slot located along the roof top vent of Bus A
Figure 15: Map view of the on-road testing path in Riverside, CA
Figure 16: Wheel-based bus speed profiles during on-road baseline stop-and-go test, on road
Figure 17: Schematic of Bus A cabin with images and locations of the aerosol generator
Figure 18: Average particle number size distributions by size inside Bus B cabin with AC fans
Figure 19: Filter efficiency versus particle size for all the testing conditions
Figure 20: Bus A test bus air velocity measurement at the internal center of the duct
Figure 21: Air velocity trends with measurements at internal center points throughout Bus A
Figure 22: Air velocity measured at internal center points throughout Bus A test bus duct
Figure 23: Air velocity measured at the outlet of vertical vents throughout Bus A test bus duct
Figure 24: Air velocity measured at the outlet of lateral vents through Bus A test bus duct
Figure 25: Non-linear regression fit of Test #04 based on CO2 decay inside Bus B cabin
Figure 26: Non-linear regression fit of Test #06 based on CO2 decay inside Bus B cabin
Figure 27: Non-linear regression fit of Test #07 based on CO2 decay inside Bus B cabin
Figure 28: Non-linear regression fit on Test #10 based on CO2 decay inside Bus A cabin
Figure 29: Non-linear regression fit on Test #11 based on CO2 decay inside Bus A cabin
Figure 30: Non-linear regression fit on Test #12 based on CO2 decay inside Bus A cabin
Figure 31: Non-linear regression fit on Test #13 based on CO2 decay inside Bus A cabin
Figure 35: On-road stop-and-go test CO2 concentration and speed profile with aerosol
Figure 35: PM arrival time, max PM, and ½ max PM from a sensor measurement inside bus
Figure 36: Test # 1 Particle concentration removal rate after aerosol generator is turned off
Figure 37: Test #2 Particle concentration removal rate after aerosol generator is turned off
Figure 38: Test #3 Particle concentration removal rate after aerosol generator is turned off
Figure 39: Test #9 Particle concentration removal rate after aerosol generator is turned off and
Figure 40: Test #10 Particle concentration removal rate after aerosol generator is turned off
Figure 41: Test #11 Particle concentration removal rate after aerosol generator is turned off
Figure 42: Test #12 Particle concentration removal rate after aerosol generator is turned off
Figure 45: Experimental setup used for the isolated ventilation system study
Figure 46: Line used to analyze the velocity and pressure across the length of the ventilation
Figure 47: Actual velocity distribution profile of the bus ventilation system
Figure 48: Pressure profile across the length of the ventilation system
Figure 49: Modified ventilation system with the equivalent inlet volume flow geometry
Figure 50: Lines used for the upper and lower longitudinal xy-plots to determine mesh independence
Figure 51: Automatic mesh level 1
Figure 52: Automatic mesh level 2 and 3
Figure 53: Automatic mesh level 4 with the added local mesh
Figure 54: Mesh study results from the lower longitudinal xy-plot
Figure 55: Mesh study results from the upper longitudinal xy-plot
Figure 56: The 11 rows of the bus and the definition of their boundaries
Figure 57: Test matrix of the different conditions applied for each case study
Figure 58: Visual representation of how the bus is separated into rows
Figure 59: Air velocity in the x-direction for cases 1 and 3
Figure 60: Fluid flow through a cross section of the bus for cases 1 and 3
Figure 62: Air velocity in the x-direction for cases 2 and 4
Figure 63: Fluid flow through a cross section of the bus for case 2 and 4
Figure 65: Air velocity in the x-direction for cases 5 and 7
Figure 66: Air velocity in the y-direction located on the driver side of the bus for cases 5 and 7
Figure 67: Fluid flow through a cross section of the bus within row 2 for cases 5 and 7
Figure 69: Air velocity in the x-direction for cases 6 and 8
Figure 70: Air velocity in the y-direction for cases 6 and 8
Figure 71: Influence of thermal plume on the fluid flow surrounding the mannequins for cases 6 and 8
Figure 73: Air velocity in the x-direction for cases 9 and 11
Figure 74: Air velocity in the y-direction for cases 9 and 11
Figure 75: Cross sectional view of an air barrier and two distinct recirculation zones in cases
Figure 77: Air velocity in the x-direction for cases 10 and 12
Figure 79: Fluid flow through a cross section of the bus within row 10 for cases 10 and 12
Figure 80: Air velocity in the y-direction for cases 10 and 12
Figure 82: Air velocity in the x-direction for cases 13 and 15
Figure 83: Air velocity in the y-direction for cases 13 and 15
Figure 84: Fluid flow through a cross section in the rear of the bus for cases 13 and 15
Figure 85: Relationship between the particles absorbed versus particles removed for cases 13 and 15
Figure 86: Air velocity in the x-direction for cases 14 and 16
Figure 87: Air velocity in the y-direction for cases 14 and 16
Figure 88: Fluid flow through a cross section in the rear of the bus for cases 14 and 16
Figure 89: Relationship between the particles absorbed versus particles removed for cases 14 and 16
Figure 90: Air velocity in the x-direction for cases 17 and 19
Figure 91: Air velocity in the y-direction for cases 17 and 19
Figure 92: Fluid flow through a cross section in the rear of the bus for cases 17 and 19
Figure 93: Relationship between the particles absorbed versus particles removed for cases 17 and 19
Figure 94: Air velocity in the x-direction for cases 18 and 20
Figure 95: Air velocity in the y-direction for cases 18 and 20
Figure 96: Fluid flow through a cross section in the rear of the bus for cases 18 and 20
Figure 97: Relationship between the particles absorbed versus particles removed for cases 18 and 20
Figure 98: Air velocity in the x-direction for cases 21 and 23
Figure 99: Air velocity in the y-direction for cases 21 and 23
Figure 100: Fluid flow through a cross section in the rear of the bus for cases 21 and 23
Figure 101: Relationship between the particles absorbed versus particles removed for cases 21 and 23
Figure 102: Air velocity in the x-direction for cases 22 and 24
Figure 103: Air velocity in the y-direction for cases 22 and 24
Figure 104: Fluid flow through a cross section in the rear of the bus for cases 22 and 24
Figure 105: Relationship between the particles absorbed versus particles removed for cases 22 and 24
Figure 106: The multi-slot design compared to the long-slit design of a ventilation system
Figure 107: The positioning of the barriers located between the seats of the subway model
Figure 108: Tram with no barriers (left). Tram with barriers highlighted in blue (right)
Figure 111: Probe location for air velocity in the middle of subway and face of inlet
Figure 112: Probe location for air velocity in the middle of tram and face of inlet ventilation
Figure 113: Ventilation inlet (top) and return/exit flow (bottom)
Figure 114: Air inlet locations (left) and outlet/exit locations (right)
Figure 115: Velocity lines gathered from the subway mesh study
Figure 116: Ideal mesh count velocity line from the subway simulations
Figure 117: Inlet velocity of the subway with 5.1 million mesh cells
Figure 118: Overview of all probed velocity over different mesh sizes
Figure 119: Ideal mesh sizes to use for simulation of the subway
Figure 120. Inlet velocity of the subway with 5.1 million mesh cells
Figure 121: Starting location of an initiated cough from the mouth
Figure 122: Positioning of mannequins on the subway and tram
Figure 123: Each zone of seating 1 through 15 and the corresponding length of each zone
Figure 124: Particle trajectory results for the subway car
Figure 126: A map of formula dependencies in the Aerosol Transmission Estimator spreadsheet
Figure 127: Graph of the input parameters vs. their relative influence on the risk multiplier
Figure 128: Relative Risk Multiplier for each vehicle and the regression predictor
Figure 129: Communication artifact
Figure A1: Laboratory experiment set up showing location of fan, four 25g CO2 cannisters and sensors
Figure A4: TSI AirAssure sensor #4 correlation to #1-#20 sensor average and #1-#10 sensor average
Figure A5: Test vehicle cabin CO2 experiment set up with TSI AirAssure sensors #1-#20
Figure A6: CO2 levels inside the test vehicle cabin containing a driver and passenger
Figure A8: PM2.5 concentrations each minute recorded by TSI AirAssure sensors #1 -#10
Figure A9: PM2.5 concentration after correction is applied to sensors #1- #10
Table 1: Bus A Test Vehicle Specifications
Table 2: Bus B Test Vehicle Specifications
Table 3: Coefficient Constraints of the non-linear CO2 regression analysis using MatLab2023
Table 4: Stationary test matrix using CO2 gas with conventional ventilation system
Table 7: Stationary test matrix using aerosol generator & new filter with conventional ventilation
Table 8: Test matrix using aerosol generator and CO2 canisters for on-road tests
Table 9: Air exchange rate results in hr-1 per sensor inside Bus B
Table 10: Air exchange rate results in hr-1 per sensor inside Bus A
Table 11: Air exchange rate results in hr-1 per sensor for on-road testing
Table 12: Particle arrival times per sensor inside Bus B with regular HVAC bus cabin for tests
Table 13: Particle arrival time per sensor with conventional ventilation system for Bus A tests
Table 14: Particle arrival time per sensor with parallel system for Bus A tests #10-18
Table 15: eACH for Bus B tests #1-3 & #9-12
Table 16: eACH for Bus A bus tests #1-9 & #22
Table 19: Turbulence parameter calculations to be used for the bus, the inlet vents, and for a cough
Table 20: Comparison of mesh size, mesh dimensions and simulation runtime
Table 21: Mesh cell counts along with simulation time and finishing conditions
Table 22: Dimensions of subway and tram
Table 24: Flowrate and Output Cross section Area
Table 25: k-є parameters of Subway
Table 26: k-є parameters of Tram
Table 27: Mesh settings used in each Subway simulation
Table 28: Results of the mesh study on the subway simulations
Table 29: Mesh settings used for each tram simulation study
Table 30: Average velocity and difference of the previous simulation on the tram
Table 31: Independent nature of input variables as it applies to risk of infection
Table 32. Processes that contribute to the removal of virus particles from the vehicle cabin
Table 33: A list of model input variables for the transit bus, light rail unit, and an airliner
Table 34: Input variable chosen for transit multivariable regression
Table 35: Yearly fuel cost from HVAC blowers
Table 36: Cost of upgrading bus with Forward Curved HVAC blowers
Table 37: Yearly cost of different filtration levels – MERV 8 and MERV 13
Table 38: Relative influence of each factor at nominal Bus A values
Table 39: Relative influence of each factor at nominal Siemens S70 Light Rail Unit values
Table 40: Relative influence of each factor at nominal Boeing 737-800 Airplane values
Table 41: Relative influence of each factor at nominal values
Table 42: Summary of the correlation parameters and their influence on the risk of infection
Table 43: Filtration improvement used for scenarios 1, 3 and 4
Table 44: Cost and Risk impact of changing from a MERV 8 to MERV 13
Table 45: Impact of both improving the filtration and setting the airflow
Table 46: Impact of increasing the speed of the blowers
Table 48: Summary table of the lowest investment required for a 10% improvement in risk reduction
Table 49: Relative influence of each factor at nominal values
Table 50: Comparison of select bus filter blower options
Table 51: U.S. average cost to reduce risk through increasing vehicle frequency
Table A1: Specifications of Sensirion SCD30 Sensor
Table A2: Location of TSI AirAssure sensors for the indoor aerosol test